Purification, Properties and Regulation of Glutamine Synthetase from Nitrobacter agilis
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چکیده
منابع مشابه
Regulation of glutamine synthetase. V. Partial purification and properties of glutamine synthetase from Bacillus licheniformis.
The glutamine synthetase of Bacillus licheniformis has been obtained at about 15% purity. Sucrose gradient centrifugation gave a molecular weight value of approximately 612,000. Both l- and d-glutamate can be utilized as substrates in the biosynthetic reaction, although the l isomer was five times more active. The requirement for adenosine triphosphate (ATP) can be partially replaced by guanosi...
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The chloroplastic glutamine synthetase of spinach leaves has been purified to homogeneity using affinity chromatography. This involves a tandem ;reactive blue A-agarose' and ;reactive red-A-agarose' as the final step in the procedure. This procedure results in a yield of 18 milligrams of pure glutamine synthetase per kilogram of starting material. The purity of our enzyme has been demonstrated ...
متن کاملPurification , localization , properties and regulation of glutamine synthetase from Hyphomicrobium X
The purification, location, properties and regulation of glutamine synthetase (GS) from the facultative methylotroph Hyphomicrobium X were investigated. The enzyme was purified to homogeneity by differential centrifugation, Blue Sepharose CL-6B chromatography and Sephadex G-25 gel filtration. The specific activity of the purified enzyme was 44.7 p o l min(mg protein)l . GS was cytoplasmic in lo...
متن کاملLack of distinction between Nitrobacter agilis and Nitrobacter winografskyi.
No adequate criteria were established to distinguish between Nitrobacter agilis and N. winogradskyi. However, very gentle preparative techniques permitted demonstration of flagella in N. agilis.
متن کاملPermeability of Nitrobacter agilis to Organic Compounds.
Ida, S. (Cornell University, Ithaca, N.Y.), and M. Alexander. Permeability of Nitrobacter agilis to organic compounds. J. Bacteriol. 90:151-156. 1965.-None of a variety of inorganic ions or organic compounds served as a sole energy source for the growth of Nitrobacter agilis, and the test substrates were not oxidized by either intact cells or extracts of the obligate chemoautotroph. The organic...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1984
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-130-4-959